FIGURE 6.
eNampt treatment of cholesterol-loaded macrophages increases the expression of specific cell survival proteins. Macrophages were preincubated in the absence or presence of 100 ng/ml eNampt for 24 h and then incubated with medium alone (Con) or medium containing eNampt, 50 μg/ml acetyl-LDL + 10 μg/ml 58035 (Chol), or eNampt plus acetyl-LDL/58035 for 6 h. Cell extracts were then assayed by immunoblot for the indicated cell survival protein and β-actin as a loading control.