FIGURE 1.
UV radiation increases Mi-2 expression. Immortalized human keratinocyte cell lines, 1106 (A) and 1102 (B), were treated with the indicated doses of UV radiation. 8 h after treatment, cells lysates were harvested, subjected to SDS-PAGE, and immunoblotted with antibodies specific for the indicated proteins. C, MCF7 cells were treated with the indicated doses of UV radiation. 8 h after treatment, cell lysates were analyzed for changes of Mi-2 protein expression. D, the 1106 keratinocyte cell line was treated with the indicated amounts of ionizing radiation. 8 h following treatment, cell lysates were harvested, and Mi-2 and β-actin protein expression were analyzed by immunoblot. E, the 1106 keratinocyte cell line was treated with 90 J/m2 of UV radiation and cell lysates harvested at indicated time points. Samples were analyzed for Mi-2 and β-actin expression by immunoblot.