FIGURE 4.

Methylated histone competes with MLL for WDR5 interaction. A, for competition experiments, GST-MLL228 was incubated with WDR5 in the presence of increasing amounts of unmodified, mono-, di-, and tri-methylated histone H3 (1-11 aa, 0, 5, 20, 60, 150 μm). Only mono- or di-methylated histone H3 is able to compete with MLL for WDR5 interaction. The numbers under the figure indicate the relative amounts of WDR5 bound to GST-MLL228, normalized by the amounts of GST-MLL228. B, similar amounts of competitors were used for the competition experiments.