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. 2008 Dec 16;3(12):e3942. doi: 10.1371/journal.pone.0003942

Figure 2. Sequence analysis of VH genes from selected anti-H5 HA scFv.

Figure 2

(a) Alignment of VH amino acid sequences from scFv containing the same V(D)J rearrangement as CR6323 or CR6261 with the germline IGHV1-69. Note that although multiple mutations are present in the CDR1 and CDR2 loops Phe at position 54 is conserved (Kabat numbering). scFv that were neutralizing in an IgG1 format are shown in bold (b) DNA alignment and amino acids for the HCDR2 of (b) CR6323 and (c) CR6261 with related mAbs. Note the identical codon usage and conservation of silent mutations suggesting a clonal origin.