Table 3. Monovalent affinity of Fabs measured by surface plasmon resonance against rHA.
mAb | HA | Ka (1/Ms) | Kd (1/s) | KD (M) |
CR6261 | H1 | 9.8×105 | 3.7×10−3 | 3.8×10−9 |
H5 | 2.7×105 | 1.1×10−3 | 4.1×10−9 | |
H9 | 6.8×105 | 3.7×10−3 | 5.4×10−9 | |
CR6323 | H1 | 1.4×105 | 5.4×10−4 | 3.9×10−9 |
H5 | 1.9×105 | 1.2×10−3 | 6.3×10−9 | |
H9 | 1.3×106 | 2.7×10−3 | 2.1×10−9 | |
CR6307 | H5 | 7.0×104 | 4.5×10−4 | 6.4×10−9 |