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. Author manuscript; available in PMC: 2008 Dec 5.
Published in final edited form as: Mol Carcinog. 2008 Oct;47(10):739–743. doi: 10.1002/mc.20423

Figure 2.

Figure 2

IFIXα upregulates maspin mRNA and protein. (A) Maspin protein levels in MDA-MB-468 vector control (V) and IFIXα stable clones (X-1 and X-2) were determined using immunoblot analysis. (B) Total RNA was isolated from MDA-MB-468 vector control (V) and IFIXα stable clones (X-1 and X-2) and subjected to northern blotting using IFIXα, maspin, and HDAC1 cDNA as probes. The 18S and 28S rRNAs are shown as loading controls. (C) MDA-MB-468 control (V) and IFIXα-expressing cells (X-1) were transfected with maspin or nonspecific (NS) siRNA and subjected to an invasion assay. The percent invasion relative to the control is indicated. Data are expressed as averages ± standard deviation (n = 3). *Significantly different, P < 0.001. The immunoblots of maspin and actin are shown above the graph.