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. Author manuscript; available in PMC: 2008 Dec 5.
Published in final edited form as: Nat Cell Biol. 2004 Dec 12;7(1):42–47. doi: 10.1038/ncb1207

Figure 3.

Figure 3

CLASP is required for subunit incorporation into K-fibre microtubules at their kinetochore-attached plus ends. (a) Control cell showing the re-growth (arrow) of a K-fibre after severing by laser microsurgery. (b) Similar to a but in a cell depleted of CLASP by RNAi. The severed K-fibre (arrow) does not re-grow even as the spindle gradually collapses. (c, d) K-fibres severed in dynein- or EB1-depleted cells, respectively, re-grow (arrow) at the same rates seen in control cells. Time is shown in min:sec; scale bar represents 5 μm. (e) K-fragment elongation curves after laser microsurgery for the cells shown in the previous panels. Movies of a–d can be found in the Supplementary Information.