Table 1.
Polymorphic STR markers for preimplantation HLA genotyping
| STR name | STR amplicon location | Forward primer (5′ --> 3′) | Reverse primer (5′ --> 3′) |
|---|---|---|---|
| Beyond the telomeric end of HLA-A gene | |||
| #31C | 29,644,080–29,644,266 | TGATCATGCCACTGCACAAC | GAACGAAGAACCATATACAACACAA |
| #115 | 29,719,377–29,719,795 | CAGGAAGAGGGCCTTACTTCT | AGATTGCGCCACTACACTCG |
| #32 | 29,747,874–29,748,272 | GGGGTTAGAAGTGTGCTTATG | AGCCAGAGTGACAGAGTAAG |
| #113 | 29,765,439–29,765,830 | TCACACCTTTTGCTCAATAGG | CAGCGCAACTGCACTTAAA |
| #36 | 29,806,777–29,807,098 | TCATGGATCTTATCAGCCTC | TCCCATGGTCAAGTTCTCAG |
| #66 | 29,830,699–29,831,034 | ATGAACTTGTCCTGAGAATGAAG | CTGTCCTATTTCATATGCTCAGG |
| #37 | 29,895,060–29,896,580 | AAAGCCCGCAGAGTGTATG | GCAATCAGCCAAGATAGCAC |
| #38 | 29,898,560–29,900,060 | TGCACCTCTGAAAGAGAGC | AACAAACCTGCACATCCTGC |
| #39B | 29,914,355–29,914,530 | CCAAGAAAGAAAGAACCAATAGCA | GAGCCAATTAGCCCAATAAATCAC |
| STRs within the HLA complex | |||
| Telomeric end of HLA-A gene | 30,018,310 | ||
| #43 | 30,056,037–30,056,525 | AGAGCTGGATTCTTAGAGCG | GTTAATGGATGCAGCACACC |
| #134A | 30,173,660–30,176,170 | GATAGAGCCAGACCTTGTC | CTGAACTTGATGAAAAGACCCTA |
| #150 | 30,458,074–30,458,713 | CCCCACAGTCATAAGTAAGTT | AAGATCGCACCACTGCTCT |
| #124 | 30,954,652–30,955,083 | TTGCAGTGAGCTGAGAATGC | CTTTCATAGCTCTCGCTGTC |
| #123 | 30,971,034–30,971,752 | CAGCAGACCTACCCTAAAAG | AGGAGTTTGAGAGTAGCCTG |
| #125 | 30,980,010–30,982,250 | TCCTGAGTAGCTAGGACTTC | TTGTTTGGACCCAGGAGGTC |
| #65 | 31,145,170–31,146,570 | GCTTGACTTGAAACTCAGAG | GTAGCTGTGGAAACAGTGTC |
| #62 | 31,312,809–31,313,090 | TTGCAGTGAGCCAAGATCGC | TCTCTGTTATCTCTGGGTATAG |
| #63 | 31,316,969–31,317,187 | GCAGTCACTTTCTCAGGTAC | TGACAGAACAAGGCTCCATC |
| #47A | 31,536,275–31,536,395 | TCTAGTGTCTTCTGGCCTTG | ATCAGGGAAAGGTGCTGGT |
| #153 | 31,830,794–31,831,424 | GCCATCACACCGAACTAAGT | GGGCTACAAGAGCAAAATCC |
| #101 | 31,944,353–31,944,793 | GGACATTGCTCTGACTTGAG | AGCTGAGATTGCACTGCTG |
| #103 | 32,142,664–32,143,116 | CCACTTCCTCCACTAGAATC | CGACAGAGTGAGACCTTGTC |
| #104A | 32,153,630–32,153,815 | ATTCCAGCCTGGGCAACAG | ACCACGCCTGACCACAAAG |
| #107 | 32,309,633–32,309,823 | AGGAGTTCATGACCAGCAAC | CAAGTAGCTGGTACCACAAG |
| #16A | 32,318,105–32,318,457 | GGCCTTTTTCACTTGTTTTTCTA | TCTTCTGCTCTACCCACCA |
| #109 | 32,377,514–32,378,034 | ACTCAACCCTGCTGTTGTAG | TGCATGTCCTGTGAGGTAAG |
| #72 | 32,433,900–32,435,440 | CTAGATGACGAGTTAGTGGG | CCGGTGTATCATTGATGGAC |
| #148 | 32,871,680–32,872,160 | TGGTGTGCGACTGTAATCC | TATCTCATGTTGAGAGCAGAC |
| #54 | 33,039,460–33,039,674 | GCTCAGTTCCAGTTGCTTG | GCAGTGAGCCAAGATTGCAC |
| #149 | 33,160,363–33,160,890 | TGCATTCCAGACTTGGCAAC | TCTTGGAGGGGGAAACATTC |
| Centromeric end of HLA-DPB2 gene | 33,204,869 | ||
| Beyond the centromeric end of HLA-DPB2 gene | |||
| #59A | 33,306,131–33,306,834 | AGCCTGGGTGACACAGCAA | CCTATCGCAATGTAAATGCTGTA |
| #78A | 33,309,804–33,310,002 | ATACTCAAATATTCCCCAGTTAG | TCTAGCCTGGGTGACAGT |
| #79 | 33,318,712–33,319,020 | ACAAGAGTGAAACTCCGTCTCAA | CATCACTGGCTCTCATGCAGA |
| #81 | 33,441,780–33,444,190 | CCACATAGCAAGTCTCCGTC | AGTTCAGTTCAAGCTAGGCTCT |
| #82A | 33,453,691–33,454,132 | TAGCCAAGATCGCACCACT | GGCAATATAGCAGAAACTCTGA |
| #84 | 33,556,390–33,560,840 | GGCTGTTGAATTGTGAGAGTTC | GGCAACAAGAGTGAAACTCTG |
| #85B | 33,564,553–33,564,762 | GAATCACTTGAACCTGGGAG | TTTACCGCAACCAAGCAAATG |
| #138 | 33,569,405–33,569,680 | GAGAGAGCGAGATTCTGC | GAATGCTTAGAAGATGAAGGTG |
| #86 | 33,571,119–33,571,243 | TGGAATGATGGGAAAATTATACT | AGCCAAGATCGTGCCATA |
| #92 | 33,641,898–33,642,580 | TTCTATCTGTTTTGACCTAGTGC | GAATGAGACAAATGGTGGAAG |
| #133 | 33,901,277–33,901,669 | CCCAGTGGAGACATTTTTGC | AAAATGACTGGAGGAGGTGC |
| #141 | 34,040,934–34,041,456 | GTCTCACCTCCAAACCATTG | ACAGAGTCAGACTCCGTCT |
| #144 | 34,198,287–34,198,679 | GGCACCATCCTGTTCCTCAGT | CCCTACTTCCAGTGCCTG |