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. Author manuscript; available in PMC: 2009 Jun 1.

Published in final edited form as: J Endod. 2008 Apr 25;34(6):728–734. doi: 10.1016/j.joen.2008.03.011

Phototoxicity of multi-species root canal biofilms after incubation with 25 µg/ml MB dissolved in BHI broth (A) or in PBS (B) for 10 minutes followed by treatment with red light of 665 nm (30 J/cm²) and colony-forming assay. Each bar is the mean Log₁₀ CFU levels (± Standard Error). The combination of light and MB was significantly lower than control, light alone or MB alone (A). The combination of light and MB was significantly lower than either control or MB alone (B).

Phototoxicity of multi-species root canal biofilms after incubation with 25 µg/ml MB dissolved in BHI broth (A) or in PBS (B) for 10 minutes followed by treatment with red light of 665 nm (30 J/cm²) and colony-forming assay. Each bar is the mean Log₁₀ CFU levels (± Standard Error). The combination of light and MB was significantly lower than control, light alone or MB alone (A). The combination of light and MB was significantly lower than either control or MB alone (B).