Table 1.

Relative levels of Raldh2 expression in hindlimb muscles

Slow Muscles	Raldh2	Fast Muscles	Raldh2
Sartorius	3.3 ± 2.3	Lateral adductor	29.8 ± 15.8
Medial adductor	4.8 ± 5.2	Ischioflexorius	30.0 ± 27.0
Femorotibialis internus	5.5 ± 7.7	Caudilioflexorius	34.7 ± 17.8
Anterior iliotibialis	9.7 ± 5.8	Ambiens	40.5 ± 24.7
Iliofibularis	25.9 ± 17.8	Femorotibialis externus & medialis	40.8 ± 22.3
		Posterior iliotibialis	48.9 ± 19.5
Average slow	9.9 ± 12.3	Average fast	37.6 ± 21.5

The cross-sectional area and Raldh2 in each muscle was measured from a cryosection through the mid-thigh in 9 embryos (St. 30 – 35). Raldh2 was expressed as a percentage of the muscle area, as described in Material and Methods. Values shown are the mean ± s.d. Muscles were categorized as predominantly fast or slow based on published studies (Crow and Stockdale, 1986; Rafuse et al., 1996) and analysis of sections stained with NA8, to identify slow muscle fibers (data not shown). On average Raldh2 was significantly less abundant in slow muscles (p < 001, t-test).