Table 3.
DMSO/Control | RA/Control | |
---|---|---|
(n = 6) | (n = 19) | |
Length of “fan” | 1.1 ± 03# | 1.4 ± 0.3** |
Area of “fan” | 1.0 ± 0.1# | 1.2 ± 0.2** |
The longest incursion of axons (length of “fan”) and the total arborization of axons in the crural plexus (area of “fan”) were measured in limbs in St. 23.5–25 embryos implanted with RA-soaked or control (DMSO-soaked) beads at St. 19–22.5, as described in Materials and Methods and Fig. 5B. The same number of sections was analyzed in treated and control limbs of individual embryos; values in each embryo were compared for significance with a paired t-test. Early exposure to exogenous RA significantly promoted axon growth into treated limbs
p > 0.05
p < 0.001.