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Cardiomyocyte viability following Akt siRNA transfection. Knockdown of Akt1/2 eliminated ME-induced protection against hypoxia-reoxygenation cell death in males but only partially attenuated protection in females. Conversely, knockdown of Akt3 was ineffective in eliminating ME-induced protection in males but abrogated it in females. Control siRNA = green fluorescent protein (GFP). Shaded area indicates hypoxic period (0–90 min). Data are expressed as means ± SE.
