Table 1. Primers used for genotyping and QPCR.
Primer | Sequence |
---|---|
Primers for genotyping | |
LOC387715/ARMS2 forward |
TACCCAGGACCGATGGTAAC |
LOC387715/ARMS2 reverse |
GAGGAAGGCTGAATTGCCTA |
HTRA1 forward |
CGGATGCACCAAAGATTCTCC |
HTRA1 reverse |
TTCGCGTCCTTCAAACTAATGG |
Primers for QPCR | |
HTRA1 forward |
AGCTGGGACTTCGGAACTCC |
HTRA1 reverse |
TCCGGAATTTCCATAATTGATGA |
GAPDH forward |
GGGGGAGCCAAAAGG GTCAT |
GAPDH reverse | GCCCCAGCGTCAAAGGTGGA |
Sequence of primers which were used for genotyping of LOC387715/ARMS2 (rs10490924) and HTRA1 (rs11200638) SNPs and for QPCR for assessment of HTRA1 mRNA levels.