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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: J Immunol. 2008 Mar 1;180(5):3467–3477. doi: 10.4049/jimmunol.180.5.3467

FIGURE 5.

FIGURE 5

Selective inhibition of the classical and noncanonical NF-κB pathways in HUVEC. A and B, HUVEC transduced with LZRS, LZRS-IKKαSSAA, or LZRS-IKKβK44M were either untreated or incubated with a range of concentrations of TNF (0.1, 1, and 10 ng/ml) (A) or LIGHT (1, 10, and 100 ng/ml) (B) for 30 min then lysates were analyzed by immunoblotting using anti-IκBα (upper), anti-IKKα, anti-IKKβ (two middle), or anti-tubulin (lower). C, HUVEC transduced with LZRS or LZRS-IKKβK44M were either untreated or incubated for 24 h with a range of concentrations of LIGHT (1, 10, and 100 ng/ml). D, Samples were analyzed for p100/p52 or tubulin by immunoblotting and densitometry was performed on the resulting immunoblot. E and F, HUVEC transduced with LZRS or LZRS-IKKαSSAA were treated and analyzed as described for C and D. Data shown are from a single experiment representative of at least three performed using separate transduced EC cultures.