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. Author manuscript; available in PMC: 2008 Dec 5.
Published in final edited form as: J Mol Biol. 2007 Feb 22;368(3):729–741. doi: 10.1016/j.jmb.2007.02.057

Fig. 6.

Fig. 6

The M770I mutation alters the interaction of GR with coactivators

(a) GST-pulldown assay comparing the interaction of equimolar concentrations of in vitro expressed, 35S-labeled GR WT and mutants with a glutathione agarose-bound GST-GRIP1 fragment (aa 567-1121; 2 μM). GST-GRIP1: GR complexes were separated by SDS-PAGE. Total and bound receptor concentrations were determined by phosphoimaging. Shown are the averages and standard deviations of three binding experiments performed in duplicates, as well as a representative autoradiogram. (b) DEX dose response curves for GR WT and M770I in transiently transfected F9 carcinoma cells using a TAT3 luciferase reporter. Relative luciferase units were normalized with respect to the GR WT activity in the presence of 0.1 μM DEX. Shown are the averages and standard deviations of three experiments performed in triplicates.