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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Hepatology. 2008 Nov;48(5):1497–1505. doi: 10.1002/hep.22515

Figure 4. Leptin induces RhoA GTP-ase activity in primary HSC and in LX-2 cells.

Figure 4

a) HSC were isolated from wild type and Zucker rats, cultured for 2 days, and then exposed to leptin (100 ng/ml) or AB. Rho GTP pull down assays and western blot analyses were performed to detect active RhoA. In HSC isolated from wild type rats leptin induced activation of RhoA, while in HSC from Zucker rats exposed to leptin, no increase in activation of RhoA was detected. N=3.

b) LX-2 cells were exposed to leptin in the presence or absence of JAK inhibitor AG490, or PI3 kinase inhibitor LY29002, and RhoA GTPase activity was studied. Western blot and densitometry showed that RhoA GTP-ase activity was dependent on JAK signaling as AG490 inhibited leptin-induced RhoA activity however, inhibition of the PI3K did not affect it. Mean ±SE, N=4, *p<0.01.