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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Hepatology. 2008 Nov;48(5):1497–1505. doi: 10.1002/hep.22515

Figure 5. Phagocytosis of apoptotic bodies by HSC induces JAK1 and STAT3 phosphorylation and RhoA GTP-ase activity.

Figure 5

A) LX-2 cells were cultured in serum-free medium, mock-transfected (only pCMV-XL6) or transfected with the SOCS3 construct, and 48 hours later exposed to AB for half an hour. Western blot analyses were performed to detect JAK1 and STAT3 phosphorylation. Transfection of LX-2 cells with the SOCS3 construct inhibited JAK1 and consequently STAT3 phosphorylation. B) RhoA pull-down assay was performed in SOCS3 or mock-transfected LX-2 cells after exposure to either leptin or AB. RhoA activity decreased in SOCS3-transfected cells compared to control (mock transfected cells) after leptin, and especially after AB treatment.