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Membrane binding of AH R25C-Akt1 domain. An AH R25C-Akt1-GFP construct was used to generate the transgenic Xenopus (AH-GFP). Tadpoles were dark-adapted overnight (A) or dark adapted overnight and then light-adapted for 30 min (B). Protein localization was visualized using the endogenous fluorescence of GFP. Experiments were done using at least 5 tadpoles for each group, and representative images are shown.
