Table 2.
Lateral diffusion of bodipy PC in unpatterned di12:0 PC supported membranes incubated with SUVs composed of di15:0 PC for 30 min followed by extensive washing with buffer.
| Unpatterned supported bilayer composition | SUV composition | Temperature (°C) | D ± SD (× 10−9 cm2 s−1) | αa |
|---|---|---|---|---|
| di12:0 PC + 0.5 mol% bodipy PC | di15:0 PC + 0.5 mol% diI-C16 | 25 (n = 20) | 4.6 ± 0.9b | 0.79 ± 0.06 |
| 37 (n = 18) | 7.9 ± 2.9c,d | — | ||
| 25 (n = 18) | 4.5 ± 1.8b | 0.78 ± 0.09 | ||
|
| ||||
| di12:0 PC + 0.5 mol% bodipy PC | di15:0 PC − 0.5 mol% diI-C16 | 25 (n = 22) | 5.8 ± 1.6b | 0.81 ± 0.08 |
| 37 (n = 20) | 6.8 ± 2.8c,d | — | ||
| 25 (n = 17) | 6.1 ± 1.7b | 0.79 ± 0.08 | ||
|
| ||||
| di12:0 PC + 0.5 mol% bodipy PC | —e | 25 (n = 22) | 7.1 ± 2.2c | — |
| 37 (n = 20) | 11.1 ± 1.9c,d | — | ||
| 25 (n = 17) | 7.9 ± 2.1c | — | ||
|
| ||||
| di12:0 PC + 0.5 mol% bodipy PC | —f | 25 (n = 83) | 6.7 ± 2.1c | — |
| 37 (n = 17) | 8.9 ± 1.7c,d | — | ||
| 25 (n = 20) | 6.7 ± 2.3c | — | ||
a
α is the anomalous diffusion exponent.
b
Fit to Eq. 2.
c
Fit to Eq. 1, where m = 1.
d
F > Fcrit, as determined by single factor ANOVA, indicating a statistically significant difference from rest of the cases at 95% confidence limit.
e
30 min incubation with TBS buffer only, in place of SUV suspension, followed by extensive washing.
f
Planar bilayers processed normally with no 30 min incubation.