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. Author manuscript; available in PMC: 2008 Dec 6.
Published in final edited form as: J Biol Chem. 2006 Oct 25;281(52):40310–40320. doi: 10.1074/jbc.M603858200

FIGURE 6. Interaction of the intact M3-MR with endogenous SET in CHO and COS-7 cells.

FIGURE 6

COS-7 cells transiently transfected with empty vector (pcDNA3) or pcDNA3::M3MR (A), CHO cells stably transfected or not with the M3-MR (CHO-M3) (B) were lysed in Nonidet P-40 buffer, and the receptor was immunoprecipitated as described under “Experimental Procedures.” The membrane transfer was first probed with anti-SET antibody followed by stripping and reprobing with a polyclonalM3-MR antibody to confirm effective receptor immunoprecipitation. The input and supernatant (Supt) lanes represent 1/25 of the lysate volume used for each immunoprecipitation (IP). The data are representative of two to three experiments.