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Effects of a low-protein diet on hypothalamic Agrp gene expression. After 7 days on the low-protein diet, brains were collected, small punches of mediobasal hypothalamus were collected, and total RNA was extracted. Levels of Agrp mRNA were assessed via real-time PCR. Hypothalamic Agrp mRNA expression was significantly increased in rats exposed to 7 days of a low-protein diet compared with controls (*P = 0.002).
