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. Author manuscript; available in PMC: 2008 Dec 6.
Published in final edited form as: Am J Physiol Endocrinol Metab. 2007 Mar 20;293(1):E165–E171. doi: 10.1152/ajpendo.00675.2006.

Fig. 4.

Fig. 4

Effects of reduced amino acid availability on Agrp gene expression in vitro. The GT1–7 hypothalamic cell line was used to assess the effects of reduced amino acids on Agrp gene expression in vitro. Cells were cultured in 5, 10, 25, 40, and 100% amino acids for 16 h, with 100% representing standard DMEM. Following exposure to low amino acids, cells were harvested to assess Agrp gene expression via real-time PCR. Decreasing amino acid levels to 10 or 5% of basal resulted in significant increases in Agrp mRNA levels (*P < 0.001).