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. Author manuscript; available in PMC: 2009 Dec 5.
Published in final edited form as: Life Sci. 2008 Oct 21;83(23-24):801–809. doi: 10.1016/j.lfs.2008.09.029

Figure 2.

Figure 2

Densitometry and representative immunoblots of hypoxia inducible factor (HIF)-1α, vascular endothelial growth factor (VEGF), prolyl hydroxylases domain (PHD)-2, VHL, 26s proteasome, phospho- and total Akt, NAD(P)H subunit p47phox, phospho- and total eNOS, and MMP-9 in HUVEC exposed to hypoxia with and without simvastatin (1μmol/L). Expression of HIF-1α, VEGF, and p-Akt were increased under hypoxia and further enhanced by simvastatin. The changes of VHL and 26s under hypoxia were restored by simvastatin, while p47 and eNOS expression remained unchanged among groups. P-Akt and p-eNOS expression was assessed relative to their respective total proteins, while the others were normalized to actin, and all were expressed as ratio. *p<0.05 vs. normal, †p<0.05 vs. hypoxia