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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Cancer Res. 2008 Nov 1;68(21):8899–8907. doi: 10.1158/0008-5472.CAN-08-2568

Figure 2. NPM-ALK regulates Rho family GTPases activation through the enhancement of GEF activity.

Figure 2

A-C, wild-type TS cells were treated with 300nM ALK inhibitor CEP-14083 for 2 hours whereas inducible sh-ALK TS cells were grown in the presence of doxycycline for 84 hours. Total cell lysates were used for Rac1 (A), Cdc42 (B) or RhoA. (C) pull-down assays to detect active GTPases. Lysates were also blotted with the indicated antibodies. The results from ten independent experiments were quantified by optical densitometry and the mean fold changes are represented. Statistical analyses were performed by student's t-test. Data are represented as mean +/− SD. * p < 0.005, ** p < 0.05.