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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Cancer Res. 2008 Nov 1;68(21):8899–8907. doi: 10.1158/0008-5472.CAN-08-2568

Figure 5. Cdc42 down-modulation impairs the growth rate of ALCL cells in vitro.

Figure 5

A, ALK positive (TS and SU-DHL1) and ALK negative (MAC-1 and Jurkat) cell lines were treated for 6 (left panel) or 20 hours (right panel) with the Cdc42 inhibitor Secramine A. Percentages of apoptotic cells were measured by TMRM staining. B, inducible sh-Cdc42 TS cells were cultivated in the presence of 1 μg/ml doxycycline for 48 hours to induce EGFP expression and Cdc42 down-modulation. Cells were then mixed in 1:1 ratio with wt TS cells and the percentages of EGFP positive cells were followed over time. Data are represented as mean +/− SD. Results are representative of three independent experiments. C, EGFP TS cells were transduced with two different lentiviral contructs encoding for specific sh-RNAs against VAV1 or a control sequence and selected with puromycin for 48 hours. Cells were then mixed in 1:1 ratio with wt TS cells and the percentages of EGFP positive cells were followed over time. Data are represented as mean +/− SD. Results are representative of three independent experiments.