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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: J Immunol. 2008 Nov 1;181(9):6170–6177. doi: 10.4049/jimmunol.181.9.6170

Figure 4. Killing of human microglia by NK cells is dependent on cell-contact and NKG2D and NKp46.

Figure 4

Microglia cells were co-cultured with purified and IL-2 activated NK cells for 4 hr at an E;T ratio of 10:1. Specific cell lysis was quantified by TO-PRO-3 staining. Shown are means and SEM of TO-PRO-3+ microglia cells compared to PKH26+ microglia cells. Fig. 4A: Co-culture was performed using transwell (TR) and non-transwell plates. 10:1 vs. TR 10:1, *p=0.0492. TR: transwell. Fig. 4B: NK cells were preincubated for one hour w/o antibodies, with IgM and IgG Isotype control antibodies, anti-NKp30, anti-NKp46 or anti-NKG2D antibody. NK:MG 10:1 vs NKG2D, *** P<0.0009, NK:MG 10:1 vs NKp46, *p = 0.03. Fig. 4C: NK cells were preincubated for one hour with IgG Isotype control antibodies, anti-DNAM-1, anti-LFA-1 or anti-2B4 antibody, ** p = 0.0073. Three experiments are summarized.