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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Eur Urol. 2008 Jan 16;54(4):845–853. doi: 10.1016/j.eururo.2008.01.010

Fig. 1. [en] shRNA knock-down of epidermal growth factor receptor (EGFR), Akt-1, and MEK-1 expression in 786-0 renal cell carcinoma (RCC) cell lines.

Fig. 1

(A) Abundance of EGFR, AKT-1, and MEK-1 mRNA transcripts in respective shRNA transfectants relative to scramble control shRNA transfectants as measured via real-time PCR. β-Actin was used as an internal control for normalization of cDNA content. (B) Immunoblotting of total protein lysates obtained from 786-0 cells transfected with shRNA for the indicated genes. Actin was immunoblotted to control for sample loading in the gel (glyceraldehyde phosphate dehydrogenase [GAPDH] was used for Mek-1 because of its more favorable molecular mass). Immunoblots were quantitated for luminescence as shown in the bar graph below each blot. All experiments were performed in triplicate; results shown are representative. Asterisk indicates statistical significance at p < 0.005.