Table 10.

Accuracy results from negative control recovery experiments consisting of triplicate sample preparations at 3 spike levels (50, 100, and 200%) by LC-UV: results for AC-C-1^a

	Aconitine	Hypaconitine	Mesaconitine	Total
Low spike
Amount standard spiked, μg	2.475	2.499	2.499	7.473
Replicate 1, amount recovered, μg	2.113	2.286	2.242	6.641
Replicate 2, amount recovered, μg	2.090	2.217	2.163	6.470
Replicate 3, amount recovered, μg	2.174	2.329	2.257	6.760
Mean, μg	2.126	2.277	2.221	6.624
SD, μgb	0.043	0.057	0.051	0.146
RSD, %c	2.042	2.481	2.274	2.201
Recovery, %	85.9	91.1	88.9	88.6
Intermediate spike
Amount standard spiked, μg/g	4.949	4.998	4.998	14.945
Replicate 1, amount recovered, μg	4.376	4.863	4.750	13.989
Replicate 2, amount recovered, μg	4.297	4.761	4.700	13.758
Replicate 3, amount recovered, μg	4.617	4.965	4.793	14.375
Mean, μg	4.430	4.863	4.748	14.041
SD, μg	0.167	0.102	0.047	0.312
RSD, %	3.763	2.097	0.980	2.220
Recovery, %	89.5	97.3	95.0	93.9
High spike
Amount standard spiked, μg/g	9.898	9.996	9.996	29.890
Replicate 1, amount recovered, μg	9.460	9.871	9.883	29.214
Replicate 2, amount recovered, μg	9.696	10.031	10.146	29.873
Replicate 3, amount recovered, μg	9.375	9.792	9.756	28.923
Mean, μg	9.510	9.898	9.928	29.337
SD, μg	0.166	0.122	0.199	0.487
RSD, %	1.749	1.230	2.003	1.659
Recovery, %	96.1	99.0	99.3	98.1

^aNegative control AC-C-1 = Radix et Rhizoma Ginseng (Panax ginseng C.A. Mey.), a raw material; weight of negative control sample used = 1 g.

^bSD = Standard deviation.

^cRSD = Relative standard deviation.