Table 11.
Accuracy results from negative control recovery experiments consisting of triplicate sample preparations at 3 spike levels (50, 100, and 200%) by LC-UV: results for AC-C-2a
| Aconitine | Hypaconitine | Mesaconitine | Total | |
|---|---|---|---|---|
| Low spike
| ||||
| Amount of standard spiked, μg | 2.475 | 2.499 | 2.499 | 7.473 |
| Replicate 1, amount recovered, μg | 2.093 | 2.307 | 2.117 | 6.517 |
| Replicate 2, amount recovered, μg | 2.267 | 2.311 | 2.363 | 6.941 |
| Replicate 3, amount recovered, μg | 2.312 | 2.428 | 2.440 | 7.180 |
| Mean, μg | 2.224 | 2.349 | 2.307 | 6.879 |
| SD, μg | 0.116 | 0.069 | 0.169 | 0.336 |
| RSD, % | 5.200 | 2.927 | 7.314 | 4.881 |
| Recovery, % | 89.9 | 94.0 | 92.3 | 92.1 |
|
| ||||
| Intermediate spike
| ||||
| Amount of standard spiked, μg/g | 4.949 | 4.998 | 4.998 | 14.945 |
| Replicate 1, amount recovered, μg | 4.332 | 4.596 | 4.460 | 13.388 |
| Replicate 2, amount recovered, μg | 4.441 | 4.703 | 4.555 | 13.699 |
| Replicate 3, amount recovered, μg | 4.593 | 4.967 | 4.512 | 14.072 |
| Mean, μg | 4.455 | 4.755 | 4.509 | 13.720 |
| SD, μg | 0.131 | 0.191 | 0.048 | 0.342 |
| RSD, % | 2.942 | 4.016 | 1.055 | 2.496 |
| Recovery, % | 90.0 | 95.1 | 90.2 | 91.8 |
|
| ||||
| High spike
| ||||
| Amount of standard spiked, μg/g | 9.898 | 9.996 | 9.996 | 29.890 |
| Replicate 1, amount recovered, μg | 8.762 | 9.236 | 9.353 | 27.351 |
| Replicate 2, amount recovered, μg | 9.073 | 9.465 | 9.665 | 28.203 |
| Replicate 3, amount recovered, μg | 9.269 | 9.852 | 9.814 | 28.935 |
| Mean, μg | 9.035 | 9.518 | 9.611 | 28.163 |
| SD, μg | 0.256 | 0.311 | 0.235 | 0.793 |
| RSD, % | 2.830 | 3.271 | 2.448 | 2.815 |
| Recovery, % | 91.3 | 95.2 | 96.1 | 94.2 |
a
Negative control AC-C-2 = “Liu Wei Di Huang Wan,” a multi-ingredient dry powder extract dietary supplement; weight of negative control sample used = 1 g.