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Gradient program for the separation of aconitine, hypaconitine, and mesaconitine
| Time, min | Methanol, % | Mobile phase A, %a | Flow, mL/min |
|---|---|---|---|
| 0 | 30 | 70 | 1.0 |
| 20 | 30 | 70 | 1.0 |
| 25 | 40 | 60 | 1.0 |
| 40 | 40 | 60 | 1.0 |
| 45 | 70 | 30 | 1.0 |
Mobile phase A = 20 mM triethylamine (adjusted to pH 3 with phosphoric acid)–methanol (95 + 5).
