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. Author manuscript; available in PMC: 2008 Dec 8.
Published in final edited form as: Mol Ther. 2007 Jul 24;15(10):1856–1862. doi: 10.1038/sj.mt.6300268

Figure 5. The light chain (LC) facilitates heavy chain (HC) secretion in cis.

Figure 5

The plasmids pCB-BDD-FVIII, pCB-HC-INT, pCB-HC-INT+pCB-LC-INT (1:1), pCB-HC-N6-INT, pCB-HC-N6-INT+pCB-LC-INT (1:1), pCB-LC-INT, and mock control were transfected into 293 cells and assayed 48 hours after transfection. The secreted factor VIII (FVIII) HC was measured by enzyme-linked immunosorbent assay. The y-axes show the concentration of FVIII HC in ng/ml. Transfections were performed in quadruplicate to obtain average values. The SD is shown in the figure. BDD-FVIII, B-domain-deleted FVIII; INT, intein sequence.