Figure 2.

Western blot of nonsynaptosomal mitochondria purified from WT mouse brain. An untreated mitochondrial preparation and a mitochondrial preparation subjected to the GTP-agarose pull-down procedure were added to the lanes marked M and M*, respectively. Positive controls were commercial guinea pig liver TGase 2 and TGase 2 subjected to GTP-agarose pull down (lanes TGase 2 and TGase 2*, respectively). The amount of protein loaded was 20 μg in the lanes marked M and M* and 8 μg for the lanes marked TGase 2 and TGase 2*. NeoMarkers antibodies and the alkaline phosphatase procedure were used to detect immunopositive bands.