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. Author manuscript; available in PMC: 2008 Dec 8.
Published in final edited form as: J Cell Biochem. 2008 Oct 15;105(3):847–858. doi: 10.1002/jcb.21880

Fig. 3.

Fig. 3

The rat Igfn1 gene encodes a single RNA transcript that is induced 100-fold following denervation. A: Total RNA (15 mg) from innervated (Inn) and 5-day denervated (Den) rat lower leg muscle were separated in 1.1% formaldehyde/agarose gels and transferred to nitrocellulose membranes. The RNA blots were hybridized with a [32P]-labeled 3.4 kb cDNA probe, which includes 2.8 kb of coding sequence and 0.6 kb of the 3′ untranslated region from the eEF12BP mRNA. Subsequently, the blot was washed and exposed to film with an intensifying screen. The blot was stripped and re-hybridized with a Gadph probe. The positions of 28S and 18S ribosomal RNA are indicated. B: Expression of rat Igfn1 in innervated (Inn) and denervated (Den) muscle was measured by an RNase protection assay. Induction of the Igfn1 mRNA is evident by 3 days after denervation and maximal by 5 days after denervation. By 5 days after denervation, the rIgfn1 mRNA is ~100-fold more abundant in denervated than innervated muscle.