Figure 2.

Analysis of gene expression from synDNA™ constructs in cultured human cells. A549 cells transfected by electroporation were lysed and crude lysates were used in immunobloting experiments, as described in the Materials and Methods. (A) Proteins were blotted with goat anti-H5N1 polyclonal serum. H5 protein can be detected as full-length HA (HA₀; 72 kDa) and as a proteolytically cleaved component, HA₁ (55 kDa); (B) Transferred proteins were blotted with affinity purified rabbit anti-NA antibody raised against the 15 amino acids in the C-terminal region of the NA protein (Biodesign Int.). A ~51 kDa protein was detected in crude lysates from H5N1 or N1 synDNA™ transfected cells but not H5 synDNA™ alone.