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. Author manuscript; available in PMC: 2008 Dec 8.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2007 May 3;27(8):1760–1767. doi: 10.1161/ATVBAHA.107.144881

Figure 4.

Figure 4

Western blot of the NonO expression in HASMCs. A, Expression of the NonO protein in HASMCs treated by 100 ng/mL TNF-α for 8 hours with or without JNK inhibitor. B, HASMCs were treated with NonO specific siRNA with or without TNF-α treatment. After the treatment, total cellular proteins were extracted and measured using Bradford method. Equal amount of proteins were electrophoresized in 8% SDS-PAGE gel. The NonO band was detected with anti-human NonO antibody and anti-rabbit IgG-HRP conjugate using ECL chemiluminescent detection method.