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. Author manuscript; available in PMC: 2008 Dec 8.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2007 May 3;27(8):1760–1767. doi: 10.1161/ATVBAHA.107.144881

Figure 5.

Figure 5

Effects of NonO and hnRNP-K on TNF-α–mediated P4Hα1 promoter suppression. A, Expression of NonO or hnRNP-K siRNA was silenced by transfection with NonO- and hnRNP-K–specific siRNAs at the same time that pGL3-P4Hα1 promoter vectors were transfected to HASMCs. Twenty-four hours after the transfection, cells were treated with or without 100 ng/mL TNF-αfor 8 hours. Luciferase mRNA levels measured by real-time RT-PCR were used to evaluate the effects. B, Expression of NonO or hnRNP-K was silenced by specific siRNA in HASMCs with no simultaneous pGL3 transfection. Twenty-four hours after the siRNA transfection, HASMCs were treated with or without 100 ng/mL TNF-αfor 8 hours. The levels of P4Hα1 mRNA were measured and compared among different conditions. ANOVA was used for comparisons among groups, and Bonferroni post hoc analysis was used to test between-group differences. **P<0.01 for TNF-αtreatment vs all other conditions except cells treated with TNF-α+hnRNPk siRNA. There was no statistical difference between controls and cells treated with NonO siRNA alone, TNF-α+NonO siRNA, and hnRNPK siRNA alone.