Skip to main content
. Author manuscript; available in PMC: 2009 Nov 15.
Published in final edited form as: Cancer Res. 2008 Nov 15;68(22):9394–9403. doi: 10.1158/0008-5472.CAN-08-2815

Figure 1. Perifosine synergistically enhances TRAIL-induced cell death in THP-1 cells.

Figure 1

A: cells were treated for 24 hr with either single agent alone or in combination at the indicated concentrations. Cell viability was then analyzed by MTT assays. B: CI as calculated from experiments reported in A. C: Annexin V-FITC/PI staining analysis of THP-1 cells treated with either perifosine or TRAIL alone and with two drugs together for 24 hr. The numbers in the lower right quadrants correspond to the percentage of cells which are Annexin V-positive and PI-negative (early apoptotic cells). D: western blot analysis demonstrating Ser 473 p-Akt and total Akt levels in THP-1 cells treated with increasing concentrations of perifosine for 16 hr. β-tubulin served as loading control.