Skip to main content
. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Mol Cancer Ther. 2008 Aug;7(8):2528–2535. doi: 10.1158/1535-7163.MCT-08-0083

Figure 2.

Figure 2

Figure 2

Ability of adenoviral endoplasmic reticulum (ER) targeting mda-7 vector (Ad-ER-mda7) to induce signaling along the ER stress-mediated cell death pathway. A, Flow cytometric analysis of apoptosis in A549, H1299, Seg1, Bic1, NHBE and WI-38 cells 72 hours after treatment with PBS, Ad-Luc (2500 vp/ or 24.5 moi), Ad-mda7 (2500 vp/ or 25.5 moi), Ad-ER-mda7 (2500 vp/ or 24.27 moi), Ad-Mito-mda7 (2500 vp/ or 22.1 moi), Ad-Nuc-mda7 (2500 vp/ or 26.59 moi), or Ad-Cyto-mda7 (2500 vp/ or 24 moi). Experiments involving each cell line were performed in triplicate. B. Western blot analysis of phosphorylated JNK (pJNK), JNK, phosphorylated c-Jun (p-cJun), cJun, caspase-4, phosphorylated PKR (p-PKR) and PKR protein expression in A549 and H1299 cell lysates 72 hours after treatment with Ad-Luc (2500 vp/ or 24.5 moi), Ad-mda7 (2500 vp/ or 25.5 moi), Ad-ER-mda7 (2500 vp/ or 24.27 moi), Ad-Mito-mda7 (2500 vp/ or 22.1 moi), Ad-Nuc-mda7 (2500 vp/ or 26.59 moi), or Ad-Cyto-mda7 (2500 vp/ or 24 moi). β-Actin expression was analyzed as a control.