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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: J Immunol. 2008 Jan 1;180(1):138–145. doi: 10.4049/jimmunol.180.1.138

Figure 6. Retinoid receptors involved in B lymphopoiesis.

Figure 6

(A) Expression patterns of different RAR isoforms on lymphoid progenitors. Double sorted LSK, ELP and Pro-L were subjected to real-time PCR. Results obtained for LSK with each primer set were artificially defined as 1, and numbers given on the Y axes were calculated as 2−ΔΔCT values. Sorted LSK cells were put in stromal cell free culture and treated with different retinoids for 7 days and subject to flow cytometry, total cell yield (B) and B lineage lymphoid cell yield (C) were calculated. ATRA is all trans retinoic acid, a ligand for RARα, RARβ and RARγ; 13Cis RA is 13-cis- retinoic acid, a ligand specific for RARα and RARγ; AM580 is a ligand specific for RARα; TTNPB is a ligand for RARα, RARβ and RARγ; AC 41848 is a specific ligand for RARγ; 9Cis RA is 9-cis-retinoic acid, a ligand for RARα, RARβ, RARγ, RXRα, RXRβ and RXRγ. All data are reported as means ± S.D. for n= 3. **, p < 0.01 (Student's t-test). The results shown are representative of three independent experiments.