Figure 4. Mouse AID “phospho-mimetic” mutants.

(A) The mAID^WT, mAID^S38A and mAID^S38AT40D are represented with the mutated residues indicated. (B) Partially purified AID protein indicated were assayed for ssDNA deamination activities. (C) The RPA- and transcription-dependent dsDNA deamination activities of AID^WT and AID^S38AT40D proteins were analyzed in presence (+) or absence (−) of PKA as described (Basu et al., 2005). (D) CSR activities of mAID^WT, mAID^S38A and mAID^S38At40D mutants in ex vivo complementation assay. Assays are presented in the same format as corresponding assays in Fig. 2, 3 and Supp. Fig.6). In (B), (C) and (D), values represent the mean from three experiments and the error bar represent the standard deviation from the mean.