Skip to main content
. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Mol Cancer Ther. 2008 Apr;7(4):905–914. doi: 10.1158/1535-7163.MCT-07-0515

Figure 5.

Figure 5

Effects of UBE1L on ISG15ylation of PML/RARα. BEAS-2B cells were individually transfected with PML/RARα containing expression constructs. Indicated groups were cotransfected with or without UBE1L. Following transfection, respective groups were treated with either ALLN (100 μmol/L) or vehicle (DMSO) for 4 (A and B) or 6 (C and D) additional hours. Immunoprecipitation followed by immunoblot analyses were done as described in Materials and Methods. The filters were probed with either a monoclonal anti-HA antibody to detect PML/RARα (A and C) or a monoclonal anti-ISG15 antibody to detect ISG15ylated forms of PML/RARα (B and D). Open arrows, positions of unconjugated PML/RARα proteins; closed arrows, positions of post-translational modification products of PML/RARα; Control, whole-cell lysates of COS-1 cells transfected with PML/RARα serve as a signal for unconjugated PML/RARα protein. These experiments established that UBE1L confers ISG15 conjugation to PML/RARα with subsequent repression of this protein.