Table 3.
macFoxp1tg macrophages and mice have diminished response to LPS in vitro and in vivo
| Cytokine, pg/mL | Wild-type | macFoxp1tg | % reduction | P |
|---|---|---|---|---|
| In vitro macrophages | ||||
| Unstimulated IL-1β | 3.1 ± 4.2 | 9.6 ± 6.3 | .13 | |
| LPS-stimulated IL-1β | 374 ± 162 | 198 ± 34 | 47 | .018 |
| Unstimulated TNF-α | 590 ± 144 | 348 ± 256 | .16 | |
| LPS-stimulated TNF-α | 10 287 ± 2907 | 7752 ± 1775 | 25 | .057 |
| Unstimulated IL-12 | ND | ND | ||
| LPS-stimulated IL-12 | 34.5 ± 15.7 | 9.7 ± 12.9 | 72 | .044 |
| Unstimulated IFN-γ | 15.1 ± 4.4 | 13.8 ± 3.9 | .43 | |
| LPS-stimulated IFN-γ | 803 ± 415 | 226 ± 93 | 72 | <.001 |
| Unstimulated M-CSF | ND | ND | ||
| LPS-stimulated M-CSF | 14.1 ± 4.9 | 4.5 ± 1.6 | 68 | <.001 |
| Unstimulated MCP-1 | ND | ND | ||
| LPS-stimulated MCP-1 | 3588 ± 794 | 2163 ± 635 | 40 | <.001 |
| In vivo LPS challenge | ||||
| Baseline IFN-γ | 433 ± 180 | 474 ± 51 | .64 | |
| 6 h post-LPS IFN-γ | 4453 ± 1951 | 2291 ± 1050 | 48 | .034 |
| Baseline IL-1β | 30.1 ± 41.8 | 47.6 ± 42.3 | .58 | |
| 6 h post-LPS IL-1β | 1464 ± 201 | 1047 ± 58 | .52 | |
| Baseline MCP-1 | 120 ± 19 | 69 ± 55 | .25 | |
| 6 h post-LPS MCP-1 | 216 ± 28 | 177 ± 56 | .11 |
Thioglycolate-elicted peritoneal macrophages from wild-type and macFoxp1tg mice were stimulated with LPS (10 μg/mL) overnight and cultured supernatants assayed for indicated cytokines by ELISA. Data represent mean plus or minus SD from 5 individual mice per genotype. For in vivo experiments, serum cytokine levels were determined in wild-type and macFoxp1tg mice challenged with LPS. Groups of 6 mice were injected via intraperitoneal route with 200 μg LPS. After 6 hours, mice were sacrificed and serum analyzed for cytokines by ELISA. Data represent mean plus or minus SD. ND indicates not detected. Percent reduction indicated for significant values only.