Figure 3.

Deficiency in Fas, TRAIL, or Bax or overexpression of Bcl-2 and Akt cannot modulate peripheral T-cell apoptosis in recipients of allogeneic TCD-BMT. Lethally irradiated (1100 cGy) LP or (1300 cGy) C3FeB6F1 mice were transplanted either with 5 × 10⁶ TCD B6 BM (WT) or TCD BM from B6 background Fas-, Bax-, and TRAIL-deficient or Bcl-2– and Akt-transgenic mice. All recipients were analyzed on day 28 after transplantation. Values represent mean (± SEM). n = 5-15 mice per group. (A-O) Thymic cellularity, numbers of peripheral donor-derived CD4⁺ and CD8⁺ T cells, and levels of peripheral T-cell apoptosis were determined. Donor cells were defined as Ly9.1⁻ cells.