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. Author manuscript; available in PMC: 2008 Dec 8.
Published in final edited form as: Biochemistry. 2006 Dec 26;45(51):15915–15920. doi: 10.1021/bi061425m

Figure 3. Degradation of HiNF-P in HeLa cells.

Figure 3

Cells were incubated in presence of DMSO (control) or the protease inhibitor MG132 for 8 or 12 hr (A). Whole cell extracts were analyzed by western blot using specific antibodies shown on the left. MCM6 is shown to confirm equal loading as well as the Coomassie staining underneath. Specificity of the proteasome pathway was addressed by using a combination of protease inhibitors (B). HeLa cells were incubated in presence of DMSO (control), MG132 (proteasome and calpain inhibitor), Lactacystin (proteasome inhibitor) or ALLM (calpain inhibitor) for 12 hr. Whole cell extracts were analyzed by western blot using specific antibodies shown on the left. MCM6 is shown to confirm equal loading as well as the Coomassie staining underneath.