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. Author manuscript; available in PMC: 2009 Oct 28.
Published in final edited form as: Curr Biol. 2008 Sep 4;18(20):1537–1545. doi: 10.1016/j.cub.2008.08.033

Figure 3. LNv hyperexcitation disrupts nocturnal sleep.

Figure 3

(A) Average amount of sleep plotted for days 5, 7, 9 and 11 under 12:12 LD. Error bars are S.E.M. LNv hyperexcited flies (red line) experience significantly lower levels of sleep throughout the night phase compared to the pdfGAL4 driver (grey line) and controls expressing non-conducting dORK-NC1 channel (black line). (B) Mean night time sleep (± SEM) of LNv hyperexcited flies is significantly lower than all other genotypes (One way ANOVA, F4,74 = 14.17, p < 0.001; and post-hoc multiple comparisons with Tukey’s HSD, p < 0.001). (C) Box plot of night time intensity of activity showing LNv hyperexcited flies are not hyperactive relative to the both genetic background controls and dORK-NC1 expressing flies (Kruskal-Wallis Test: H4,80 = 4.7, p = 0.31 (D) Box plot of night time sleep bout duration showing LNv hyperexcited flies experience significantly lower duration sleep episodes relative to the both genetic background controls and dORK-NC1 expressing flies (Kruskal-Wallis Test: H4,77 = 24.3, p <0.001; multiple comparisons, p < 0.01). Median, 25-75 percentile and range are indicated. (E) Sleep latency after night onset is greater in LNv hyperexcited flies compared to all other genotypes (one-way ANOVA F4,75 = 14.5, p < 0.001; Tukey’s HSD, p < 0.05).