Figure 3.

NaChBac expression in LN_V pacemaker neurons induces complex free-running behavioral rhythms with multiple superimposed periods. Double-plotted locomotor actograms and Lomb–Scargle periodograms are shown, spanning 17 d in DD of representative male progeny of the indicated genotypes after release from diurnal 12 h light/dark entraining conditions. The bar above each actogram indicates subjective day (gray) and subjective night (black), and the angled lines across the actograms indicate free-running rhythms. Periodograms determined by Lomb–Scargle analysis (Van Dongen et al., 1999) corresponding to each actogram show activity power in arbitrary units (y-axis) as a function of period in hours (x-axis), with the horizontal line across the periodograms indicating the p=0.05 statistical significance level. pdf>dORKΔ-NC control flies expressing dORKΔ-NC in the LN_Vs exhibit a single statistically significant free-running rhythm of locomotor activity. In contrast, many pdf>NaChBacflies expressing NaChBac in the LN_Vs exhibit multiple statistically significant superimposed free-running rhythms of locomotor activity, mathematically defined by periodogram peaks extending above the p = 0.05 threshold and separated by intervening regions that dip below the p = 0.05 threshold.