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. 2008 Dec 17;3(12):e3959. doi: 10.1371/journal.pone.0003959

Figure 4. Smad7 inhibits DN-RhoA induced apoptosis.

Figure 4

Cells grown in 10%FBS were transfected with a GFP overexpressing vector or with a vector that overexpresses DN-RhoA fused to GFP (N19-RhoA-GFP). Some cells were cotransfected with a Smad7 overexpressing vector. After 48h of transfection apoptosis was evaluated. Figure a shows representative cells (confocal microscopy). Cells were fixed and stained with propidum iodide and mounted in mowiol. Apoptotic nuclei are bright, condensed and present nuclear bodies in the cytoplasm (arrows) and transfected cells appear in green. Figure b shows the percentage of hypodiploid cells expressed as mean±SEM of 4 experiments. *p<0.05 vs. control ζ p<0.05 vs. DN-RhoA transfected cells.