Skip to main content
. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Structure. 2008 Nov 12;16(11):1689–1701. doi: 10.1016/j.str.2008.09.005

Table 1.

Binding affinities of NBD-556 and JRC-II-191 to different gp120 variantsa

HIV-1 YU2 gp120 glycoprotein Kd (μM)
NBD-556 JRC-II-191
w.t. 3.7 0.80
D368A 2.2 0.72
S375A 0.31 0.14
D368A/S375A 0.26 0.083
S375W NB NB
I424A NB NB
W427A NB NB
V430A 3.5 1.0
M475A NB NB
a

The binding experiments were carried out by isothermal titration calorimetry at 25°C, as described in Methods. NB - no detectable binding.