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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: J Cell Physiol. 2008 Dec;217(3):569–576. doi: 10.1002/jcp.21565

Figure 5. AR proteolytic activity in cytosolic fraction from celastrol-treated apoptitic PC-3 cells binds to calmodulin (CaM).

Figure 5

Equal amount of cytosolic fractions (200 µg) prepared from PC-3 cells treated without (non-Apop) or with (Apop) 5 µmol/L celastrol for 20 hours were incubated overnight with calmodulin (CaM)-, aprotinnin (Apro)-, trypsin inhibitor (Try Inh)- and Protein A (Pro A)- agarose beads, and the proteins pulled down with agarose beads as described in Materials and Methods were incubated with AR-containing LNCaP cell extracts (A) or rhAR (B) for 1 hour. Residual AR and cleaved calpain small subunit (Capain Sm) in the incubations were determined by Western blot analysis using AR (N20) and calpain small subunit antibodies.