Confocal imaging of F- and G-actin double-staining of Clone 9 rat hepatocytes showing fluorescence (left column) and transmission (right column) images (control and incubated with 200 nM PTXs or Lat A). F- and G-actin were labeled with Oregon Green 514 phalloidin and Texas Red DNase I, respectively. Panels (a) and (b) show photographs from control cells, while panels (c) and (d) are from cells treated with PTX-1, (e and f) with PTX-2, (g and h) with PTX-2SA, (i and j) with PTX-11, and (k and l) with Lat A. Images are representative of three independent experiments. Scale bar=50 μm.